Publications

2026

(46) Keisuke Nagao, Tatsuya Osaki, Sho Toyonaga, Chae Gyu Lee, Emmanuel Vargas Paniagua, Emily Crespin Guerra, Scott Machen, Jacob L. Beckham, Mriganka Sur, Daniel Griffith Anderson, Robert J. Macfarlane, Polina Anikeeva,” CHARIOT-AAV: Conjugation of diverse vectors to adeno-associated viruses for delivery of large genes”, bioRxiv, 2026,

https://doi.org/10.64898/2026.04.13.718054

(45) Tatsuya Osaki, Chloe Delepine, Yuma Osako, Devorah Kranz, April Levin, Charles Nelson, Michela Fagiolini, Mriganka Sur. “Early differential impact of MeCP2 mutations on functional networks in Rett syndrome patient-derived human cerebral organoids”. Nature Communications,  2026. https://doi.org/10.1038/s41467-026-71458-0

(44) Tatsuya Osaki*, Zhengpeng Wan, Koji Haratani, Ylliah Jin, Marco Campisi, David A. Barbie, Roger Kamm, and Mriganka Sur. “miR126-mediated impaired vascular integrity in Rett syndrome”. Molecular Psychiatry, s41380-026-03492-9, 2025. https://doi.org/10.1038/s41380-026-03492-9

(43) Marco Campisi, Tatsuya Osaki , Ian Dryg, Jacqueline Wolff, Jason Weirather, Nicholas Weaver, Mubin Tarannum, Carla Stornante, Pieter Schol, Minyue Chen, Keiichi Ota, Sung Rye Park, Caroline Fahey, Yixiang Li, Sarah Shelton, Satomi Hirose, Clare Padrick, Elena Ivanova, Minh Ha, Sophie Kivlehan, Patrick Lizotte, Talal El-Zarif, Jacob Berchuck, Alan Bers, Erik H. Knelson, Valeria Chiono, Kathleen Pfaff, Matthew Freedman, F. Stephen Hodi, Michael Y. Tolstorukov, Matthew G. Oser, Michal Sheffer, Constantine Mitsiades, Roger D. Kamm, Rizwan Romee, Cloud Paweletz, Scott Rodig, David A. Barbie, Navin R. Mahadevan. “Vascular STING activation facilitates NK cell antitumor immunity in small cell lung cancer”, Cancer Cell, 2026; 44, 858-878.e16 https://doi.org/10.1016/j.ccell.2026.02.008 

2025

(42) Seunggyu Kima, Tianxin Cao, Zhengpeng Wan, Jaesang Kim, Zhuxuan Li, Legairre A.Radden II, Rakesh Santhanam, Eunkyung Ko, Tatsuya Osaki, Sarah Spitz, Hyunmin Moon, Maria Proestaki, Seokbeom Roh, Gyudo Lee, Jessie S. Jeon, Curtis R. Warren, Roger D. Kamm.“ A Microphysiological Interface of Skeletal Myobundles and Inflamed Adipose Tissue for Recapitulating Muscle Dysfunction in an Obese Microenvironment”. Advanced Healthcare Materials, e02711, 2025. https://doi.org/10.1002/adhm.202502711

(41) Tatsuya Osaki, David Lee , Xiang Zhang , Rebecca Zubajlo , Mercedes Balcells , Elazer Edelman , Brian Anthony , Mriganka Sur, and Peter So.“ Multi-photon, label-free photoacoustic and optical imaging of NADH in brain cells”. Light, Science&Appilcation, 14(264), 2025. https://doi.org/10.1038/s41377-025-01895-x

(40) Tomoki Asaba, Tatsuya Osaki, Koki Murayama, Sayuri Hamano, Tatsuto Kageyama, Junji Fukuda.“ Effects of vascular endothelial cells on the neurite outgrowth of nerve cells”. Journal of Bioscience and Bioengineering, 140(3), 2025. https://doi.org/10.1016/j.jbiosc.2025.05.010

2024

(39) Nobunari Sasaki, Mizuki Homme, Takahiko Murayama, Tatsuya Osaki, Toshiyuki Tenma, Tadaichi An, Yujiro Takegami, Tetsuo Tani, Patrick C. Gedeon, Yoshihisa Kobayashi, Israel Cañadas, David A. Barbie, Ryoji Yao, and Shunsuke Kitajima* “RNA sensing induced by chromosome missegregation augments anti-tumor immunity", Molecular Cell, vol 85(4), 2024, https://doi.org/10.1016/j.molcel.2024.11.025

(38) Satomi Hirose, Tatsuya Osaki*, Roger D. Kamm. “Polyploidy of MDA-MB-231 cells drives increased extravasation with enhanced cell-matrix adhesion”. APL Bioengineering. 9, 016105 (2025). https://doi.org/10.1063/5.0233329

(37) Tatsuya Osaki*, Tomoya Duenki. Siu Yu A.Chow, Yasuhiro Ikegami, Romain Beaubois, Timothée Levi, Nao Nakagawa-Tamagawa, Yoji Hirano, and Yoshiho Ikeuchi. “Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons. Nature Communication, 15, 2945 (2024). https://doi.org/10.1038/s41467-024-46787-7

(36) Marine A Krzisch, Bingbing Yuan, Wenyu Chen, Tatsuya Osaki, Dongdong Fu, Carrie M Garrett-Engele, Devon S Svoboda, Kristin R Andrykovich, Mriganka Sur, Rudolph Jaenisch, “The A53T mutation in α-synuclein enhances pro-inflammatory activation in human microglia”, Biological Psychiatry, 2024. https://doi.org/10.1016/j.biopsych.2024.07.011

(35) Yasuhiro Ikegami, Tomoya Duenki, Ikuma Arakaki, Ryo Sakai, Tatsuya Osaki, Satoshi Ashihara, Tsuyoshi Furushima, Yoshiho Ikeuchi “A simple and inexpensive laser dissection of fasciculated axons from motor nerve organoids”, Frontiers in Bioengineering and Biotechnology, 12, 1259138, 2024. https://doi.org/10.3389/fbioe.2024.1259138

2023

(34) Hironori Saito, Tatsuya Osaki, Yoshiho Ikeuchi, Shintaro Iwasaki, “High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS”, bio-protocol, 13 (3), 2023. https://doi.org/10.21769/bioprotoc.4602

2022

(33) Maider Badiola-Mateos, Tatsuya Osaki, Roger D. Kamm, Josep Samitier “In vitro modeling of human proprioceptive sensory neurons in the neuromuscular system”, Scientific Reports, 12 (1), 1-19, 2022. https://doi.org/10.1038/s41598-022-23565-3

(32) Hyeonyu Kim, Tatsuya Osaki, Roger D Kamm, H Harry Asada “Multiscale engineered human skeletal muscles with perfusable vasculature and microvascular network recapitulating the fluid compartments”, Biofabrication, 15 (1), 015005, 2022. https://doi.org/10.1088/1758-5090/ac933d

(31) Shunsuke Kitajima, Tetsuo Tani, Benjamin F Springer, Marco Campisi, Tatsuya Osaki, Koji Haratani, Minyue Chen, Erik H Knelson, Navin R Mahadevan, Jessica Ritter, Ryohei Yoshida, Jens Köhler, Atsuko Ogino, Ryu-Suke Nozawa, Shriram K Sundararaman, Tran C Thai, Mizuki Homme, Brandon Piel, Sophie Kivlehan, Bonje N Obua, Connor Purcell, Mamiko Yajima, Thanh U Barbie, Patrick H Lizotte, Pasi A Jänne, Cloud P Paweletz, Prafulla C Gokhale, David A Barbie  “MPS1 inhibition primes immunogenicity of KRAS-LKB1 mutant lung cancer”, Cancer Cell, 40 (10), 1128-1144. e8, 2022. 10.1016/j.ccell.2022.08.015

(30) Siu Yu A Chow, Kazuki Nakayama, Tatsuya Osaki, Maki Sugiyama, Maiko Yamada, Hirotaka Takeuchi, Yoshiho Ikeuchi “Human sensory neurons modulate melanocytes through secretion of RGMB”, Cell Reports, 40 (12), 111366 2022. https://doi.org/10.1016/j.celrep.2022.111366

(29) Siu Yu A Chow, Huaruo Hu, Tatsuya Osaki, Timothée Levi, Yoshiho Ikeuchi “Advances in construction and modeling of functional neural circuits in vitro” Neurochemical Research, 47 (9), 2022. https://doi.org/10.1007/s11064-022-03682-1

(28) Yusuke Kimura, Hironori Saito, Tatsuya Osaki, Yasuhiro Ikegami, Taisei Wakigawa, Yoshiho Ikeuchi, Shintaro Iwasaki. “Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation” RNA, 28 (6), 2022. https://doi.org/10.1261/rna.079097.122

(27) Hyeonyu Kim, Tatsuya Osaki, Roger D Kamm, H Harry Asada, “Tri‐culture of spatially organizing human skeletal muscle cells, endothelial cells, and fibroblasts enhances contractile force and vascular perfusion of skeletal muscle tissues” The FASEB Journal, 36 (8). 2022. https://doi.org/10.1096/fj.202200500R

2021

(26) Tatsuya Osaki, Kazuhiro Kashiwagi, Yuichi Shichino, Ayako Sakamoto, Madoka Nishimoto, Mari Takahashi, Mari Mito, Friedemann Weber, Yoshiho Ikeuchi, Shintaro Iwasaki, Takuhiro Ito “eIF2B-capturing viral protein NSs suppresses the integrated stress response”, Nature Communication, 12 (1), 1-12, 2021. https://doi.org/10.1038/s41467-021-27337-x

(25) Tatsuya Osaki, Zhengpeng Wan, Shunsuke Kitajima, David A. Barbie, Mark R. Gillrie, and Roger D. Kamm. “Release of motor neuron exosomes containing TDP-43 and mTNF-α near the neuromuscular junction induces skeletal muscle atrophy and reduced contractility in a 3D human model of ALS”, Sneak Peek, http://dx.doi.org/10.2139/ssrn.3581364

2020

(24) Tatsuya Osaki, Siu Yu A Chow, Yui Nakanishi, Joel Hernández, Jiro Kawada, Teruo Fujii, Yoshiho Ikeuchi, “Three-Dimensional Motor Nerve Organoid Generation.”, Journal of Visualized Experiments, 163, 2020. https://doi.org/10.3791/61544

(23) Marco Campisi, Shriram K Sundararaman, Sarah E Shelton, Erik Henry Knelson, Elena Ivanova, Israel Cañadas, Ryohei Yoshida, Tatsuya Osaki, Sharon WL Lee, Tran Thai, Saemi Han, Brandon P Piel, Sean Gilhooley, Cloud P Paweletz, Valeria Chiono, Roger D Kamm, Shunsuke Kitajima, David A Barbie, “Tumor-derived cGAMP regulates activation of the vasculature”, Frontiers in Immunology, 11, 2090, 2020. https://doi.org/10.3389/fimmu.2020.02090

(22) Sharon Wei Ling Lee, Marco Campisi, Tatsuya Osaki, Luca Possenti, Clara Mattu, Giulia Adriani, Roger D. Kamm, Valeria Chiono, “Modeling Nanocarrier Transport Across a 3D In Vitro Human Blood-Brain-Barrier Microvasculature”, Advanced Healthcare Materials, 1901486, 2020. https://doi.org/10.1002/adhm.201901486

(21) Kotaro Tsuboyama, Tatsuya Osaki, Eriko Matsuura-Suzuki, Hiroko Kozuka-Hata, Yuki Okada, Masaaki Oyama, Yoshiho Ikeuchi, Shintaro Iwasaki, Yukihide Tomari, “A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation”, PLoS Biology, 18 (3), e3000632, 2020. https://doi.org/10.1371/journal.pbio.3000632

(20) Tatsuya Osaki, Uzel Sebastien, Roger D Kamm, “On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease”, Nature Protocols, 1-29, 2020. https://doi.org/10.1038/s41596-019-0248-1

2019

(19) Sharon Wei Ling Lee, Camilla Paoletti, Marco Campisi, Tatsuya Osaki, Giulia Adriani, Roger D Kamm, Clara Mattu, Valeria Chiono “MicroRNA delivery through nanoparticles”, Journal of Controlled Release, 313(10), 80-95, 2019 (Review paper) https://doi.org/10.1016/j.jconrel.2019.10.007

(18) Dina Myasnikova, Tatsuya Osaki, Kisaki Onishi, Tatsuto Kageyama, Binbin Zhang Molino, Junji Fukuda, “Synergic effects of oxygen supply and antioxidants on pancreatic β-cell spheroids”, Scientific Reports, 9(1), 2019. https://doi.org/10.1038/s41598-018-38011-6

2018

(17) Tatsuya Osaki, Uzel Sebastien, and Roger D Kamm, “Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons”, Science Advances, 4, eaat5847, 2018. https://doi.org/10.1126/sciadv.aat5847

(16) Marco Campisi, Yoojin Shin, Tatsuya Osaki, Cynthia Hajal, Roger D Kamm “3D Self-Organized Microvascular Model of the Human Blood-Brain Barrier with Endothelial Cells, Pericytes and Astrocytes”, Biomaterials, 180, 117-129, 2018. 10.1016/j.biomaterials.2018.07.014

(15) Tatsuya Osaki, Vivek Sivathanu, Roger D Kamm “Vascularized microfluidic organ-chips for drug screening, disease models and tissue engineering”, Current Opinion in Biotechnology, 52, 116-123, 2018. (Review paper). https://doi.org/10.1016/j.biomaterials.2018.07.014

(14) Tatsuya Osaki, Jean C. Serrano, Roger D Kamm “Cooperative Effects of Vascular Angiogenesis and Lymphangiogenesis”, Regenerative Engineering and Translational Medicine, 4 (9), 1-13, 2018. https://doi.org/10.1007/s40883-018-0054-2

(13) Tatsuya Osaki, Vivek Sivathanu, Roger D Kamm “Engineered 3D vascular and neuronal networks in a microfluidic platform”, Scientific reports, s41598-018-23512-1, 2018. https://doi.org/10.1038/s41598-018-23512-1

(12) Tatsuya Osaki, Vivek Sivathanu, Roger D Kamm “Crosstalk between developing vasculature and optogenetically engineered skeletal muscle improves muscle contraction and angiogenesis”, Biomaterials, 156, 65-76, 2018. https://doi.org/10.1016/j.biomaterials.2017.11.041

2017

(11) Tatsuya Osaki, Yoojin Shin, Vivek Sivathanu, Marco Campisi, Roger Kamm, “In vitro microfluidic models for neurodegenerative disorders”, Advanced healthcare materials, DOI: 10.1002/adhm.201700489, 2017. https://doi.org/10.1002/adhm.201700489

(10) Tatsuya Osaki, Tatsuto Kageyama, Yuka Shimazu, Dina Mysnikova, Shintaro Takahashi, Shinichi Takimoto, Junji Fukuda, “Flatbed epi relief-contrast cellular monitoring system for stable cell culture”, Scientific Reports, 7, 1897 (2017). https://doi.org/10.1038/s41598-017-02001-x

(9) Junko Enomoto, Tatsuto Kageyama, Tatsuya Osaki, Flavia Bonalumi, Francesca Marchese, Alfonso Gautieri, Elena Bianchi, Gabriele Dubini, Chiara Arrigoni, Matteo Moretti, Junji Fukuda, “Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM”, Scientific Reports,7, 2017. https://doi.org/10.1038/srep43375

-2016

(8) Junko Enomoto, Naoto Mochizuki, Katsumi Ebisawa, Tatsuya Osaki, Tatsuto Kageyama, Dina Myasnikova, Tadashi Nittami, Junji Fukuda, “Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates”, Regenerative Therapy, 3, 24-31, 2016. https://doi.org/10.1016/j.reth.2015.12.003

(7) Tatsuya Osaki, Takahiro Kakegawa, Tatsuto Kageyama, Junko Enomoto, Tadashi Nittami, Junji Fukuda, “Acceleration of vascular sprouting from fabricated perfusable vascular-like structures”, PloS one, 10(4), e0123735, 2015. https://doi.org/10.1371/journal.pone.0123735

(6) Tatsuto Kageyama, Takahiro Kakegawa, Tatsuya Osaki, Junko Enomoto, T Ito, Tadashi Nittami and Junji Fukuda, “Rapid engineering of endothelial cell-lined vascular-like structures in in situ crosslinkable hydrogels”, Biofabrication , IOP science, 6, 2, 025006, 2013. https://doi.org/10.1088/1758-5082/6/2/025006

(5) Tatsuya Osaki, Takahiro Kakegawa Naoto Mochizuki, Junji Fukuda, “Fabrication of perfusable vasculatures by using micromolding and electrochemical cell transfer”, IEEE EMBC, 6655-6658, 201310.1109/EMBC.2013.6611082. https://doi.org/10.1109/EMBC.2013.6611082

(4) Junji Fukuda, Shintaro Takahashi, Tatsuya Osaki, Naoto Mochizuki and Hiroaki Suzuki, “Processing of nanolitre liquid plugs for microfluidic cell-based assays”, Sci. Technol. Adv. Master, 13 2012. https://doi.org/10.1088/1468-6996/13/6/064201

(3) Tatsuya Osaki, Takahiro Kakegawa, Hiroaki Suzuki, Junji Fukuda Electrical Detachment of Cells for engineering Capillary-like structures in a Photocrosslinkable hydrogel, IEEE EMBS, 2451-5254, 2011. https://doi.org/10.1109/IEMBS.2011.6090681

(2) Nasser Sadr, Mojun Zhu, Tatsuya Osaki, Takahiro Kakegawa, Yunzhi Yang, Matteo Moretti, Junji Fukuda, Ali Khademhosseini,” SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures”, Biomaterials, 32, 7479-7490, 2011. https://doi.org/10.1016/j.biomaterials.2011.06.034

(1) Naoto Mochizuki, Takahiro Kakegawa, Tatsuya Osaki, Nasser Sadr, Nezamoddin N Kachouie, Hiroaki Suzuki, Junji Fukuda, “Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide”, Journal of tissue engineering and regenerative medicine, 10.1002/term.519, 2011. https://doi.org/10.1002/term.519